It will also chime at midnight to welcome 2021.
The bell, which weighs 13.7 tonnes, last rang on November 11 to mark Armistice Day.
This year Londoners have been urged to stay away from Westminster on New Year’s Eve due to coronavirus restrictions.
The bell will be tested in the days leading up to New Year’s Eve to ensure it can produce its 12 bongs when the clock strikes midnight.
One of the tests will be an hour before midnight, which coincides with the end of the Brexit transition period.
Before the pandemic, Brexiteers tried and ultimately failed to get the bell to ring in the moment the UK left the European Union on January 31.